OUR TECHNOLOGY
Transform
your research
How it works
Mera System and Bioconsumable Plate
Mera System
Overview
Hooke Bio’s Mera has the ability to culture, drug and stain 3D microtissues at higher throughput than existing technologies while analysing physiological metrics relevant to drug functionality and toxicity in real time through a fully automated integrated fluorescent microscope.
To benchmark Mera against existing 3D culture systems, HepG2 liver microtissues (spheroids) were cultured on the bioconsumable plate in Mera and compared to microtissues grown on a microplate in an incubator. The microtissues were stained with Calcein AM (response shown in green indicating good viability) and EthD-1 (response shown in red indicating low levels of apoptosis). In addition to viability, the recirculation fluidics can support inter-microtissue signalling mimicking the fluidic systems in the human body.
In house image analysis software was developed to facilitate reproducible measurement of physiological responses in microtissues. Data generated on the Mera system demonstrates that HepG2 microtissues exposed to Acetaminophen (APAP, also known as paracetamol) results in similar levels of cell death in comparison to microtissues cultured in microplates also exposed to APAP.
- Microphysiological system (MPS)
- Fully automated integrated system
- Precise feeding and dosing flow control
- Communication via fluidic interactions
- Recirculation – inter-mictotissue communication
- On system imaging
Currently only 1 in 10 drugs make it through clinical trials to market due mostly to poor preclinical models. Based on recent literature, 3D microtissues which are also known as organoids/spheroids, give more physiological responses than 2D models and may be suitable replacement for animal models in preclinical drug testing.
Bioconsumable plate
Speak to our teamOVERVIEW
The Hooke Bio system comes in two parts, a Bioconsumable plate and the Mera platform. Our system has the ability to feed, wash, drug, and/or stain microtissues within the bioconsumable plate and has the ability to maintain and treat up to 800 microtissues simultaneously.
Mera supports fluidic recirculation. mimicking conditions within the human body and its integrated optics to allow for live microtissues metric assessment.
- In vivo type environment
- Parallel culture of different microtissues
- Inter-tissue interaction
- SBS/ANSI format
SIGN UP